The Process of Proving Infidelity

Boston Paternity uses the newest DNA technology to prove infidelity. Boston Paternity's infidelity DNA testing process has three parts.

• Semen Screen

First, our DNA Laboratory experts screen a sample for semen or vaginal fluid. The sample can be from an undergarment, bed sheet, blanket, clothing, condom, or other suspicious article. Articles containing suspicious stains can be rapidly and reliably screened by Boston Paternity's accredited forensic laboratory for the presence of semen utilizing a combination of three techniques: ultraviolet illumination, prostrate specific antigen (p30) and microscopy. Even if a man is vasectomized, his semen can still be detected.

Ultraviolet Examination

Ultraviolet (UV) light is a popular tool in many forensic investigations for the presumptive identification of body fluids on a variety of substrates. Materials such as semen, saliva, perspiration and vaginal secretions are naturally fluorescent under UV light which offers a discriminating method for locating these stains which might otherwise be invisible to the naked eye. Once the precise location of the stain isdetermined, presumptive testing can be conducted utilizing the p30 and microscopy techniques.

Prostate Specific Antigen (p30)

Prostate specific antigen, also known as p30, is secreted into seminal fluid at concentrations ranging from 200,000 to 5.5 million nanograms per milliliter. The p30 test is an extremely sensitive presumptive technique that allows for the low level detection of seminal fluid in stains. The technique is extremely powerful because it can confirm the presence of semen even in samples from sterile or vasectomized men.

Microscopy

Sperm heads can be accurately identified based on their morphological characteristics via microscopy.

• DNA Profile

When the article shows a positive for biological fluid the next step is to obtain a viable DNA profile from the sample. The laboratory extracts DNA from the sample and produces a DNA profile. This profile can be compared to your DNA to determine if infidelity is taking place.

Biological stains resulting from sexual activity often contain a mixture of both male and female DNA. The female DNA usually originates from epithelial cells from the vaginal wall, the mouth or skin, while the male DNA originates from seminal fluid. If the DNA profile of the stain shows the presence of a male, it will be compared against your reference cheek swab. If the male DNA in the stain does not match that of your DNA, the results indicate that the semen stain is from another man. In most cases, a DNA profile can be obtained from a vasectomized man.

• DNA Comparison

After BP has extracted usable DNA from the suspicious article the forensic experts will move to the final stage of infidelity testing. The DNA profile from the stain is compared to your reference DNA sample. A reference sample is your DNA sample in the form of a cheek swab. If the reference sample does not match the DNA profile from the suspicious article, then infidelity is confirmed.

Infidelity Test Results

• Semen Screen

The results of a semen screen will come back positive or negative within 5 business days. Boston Paternity can provide confidential results via telephone, email, mail, or fax. View our Semen Screen Results Report.

• DNA Profile

When the results of a semen screen are returned positive the next step is to attempt to extract DNA from the suspicious article. The results for the DNA profile will return with either a complete DNA profile, a partial DNA profile, or no DNA profile. View our Infidelity DNA Profile Results Report. The results will depend on the amount of usable DNA that is present on the article. Boston Paternity uses the best possible technology to obtain a complete DNA profile, which is accomplished in about 95% of infidelity cases.

• DNA Comparison

The final step of an infidelity DNA test is to compare a reference sample to the DNA profile of the suspicious sample. This final step is your definitive proof of a cheating wife or a cheating husband. View a sample Infidelity DNA Test Results Report.

Choose Boston Paternity

Catching a cheating wife or a cheating husband is not easy physically or emotionally. Infidelity is the cause of at least 20% of divorces and can cause significant heartache. Boston Paternity has infidelity experts on staff ready to help you find a solution to your suspicions about a cheating husband or a cheating wife. Call Boston Paternity today to confirm your suspicions. Boston Paternity is the DNA solution to infidelity.